Characterizing the change in expression of the CH protein in the mouse cerebellum labrats

Hey I really need help but I am running out of the time ! would you help me please answering this homework

The question is characterizing the change in expression of the CH protein in the mouse cerebellum.

with a series of formalin-fixed histology sections of cerebellum of the C57/BL6J mice (treated with and without ibuprofen, n=5). Within your toolbox /refrigerator are the following reagents:

• Goat monoclonal (anti-CH) and rabbit polyclonal (anti-CH) antibodies have been developed, which can both recognize the crackhead (CH) protein. (The goat Ab is only suitable for immunolocalization in FFPE tissues; the rabbit Ab is only suitable for Western blotting.)

• Anti-Rabbit IgG (made in Goat) • Avidin Biotin Complex Reagent (ABC reagent, Vector Laboratories) • Normal Sheep Serum • Normal Goat Serum • Phosphate buffered saline • Avidin • Protein A • Biotin-HRP • Anti-Goat IgG (made in Rabbit) • Bioinylated Anti-Mouse IgG (made in Goat) • Biotinylated Anti-Rabbit IgG (made in Rat) • Anti-Mouse IgG (made in Rabbit) • FITC –labeled anti-Mouse IgG (made in Swine) • TRITC-labeled anti-Rabbit IgG (made in Rat) • Diaminobenzidine • Normal Horse Serum

Design a strategy to complete the assignment to identify the cellular localization of CH in the mouse brain. Identify the steps that will be taken, and the reagents used to successfully validate a change in CH expression in situ ?

Submitted April 30, 2016 at 06:09AM by shadowplay2016 http://ift.tt/1pQv5qF labrats