Hello All,
I am looking for any advice that you might have to offer on the propagation of two gentians: Gentianopsis crinita and Gentiana andrewsii. There are few published articles that I could find on the exact methods of stratification and germination. I know that gentians can be challenging and often have low germ rates (>50%). Using a mixture of research articles and anecdotal information from blogs/websites I have compiled the following plan for propagation. I was hoping that you might help to confirm or deny the likelihood of decent germination rates using the protocol. This work is being done for an independent study on rare plant conservation and is intended to produce plugs of these species which are becoming increasingly rare in our region for a spring reintroduction into several fen wetlands.
Propagation "Protocols" 1. Surface Sterilization (Is this necessary or worth it?): Bleach diluted to 5% sodium hypochlorite with a few drops of Tween 20, seeds rinsed in solution using a shaker for 5-10 mins and then rinsed 3x in ddH2O for 5-10mins each. Then straight into next step. 2. Stratification: Presoak seeds in ddH2O for 6 hours. Remove seeds and keep moist on damp paper towels (should these be treated with a fungicide?) in a plastic bag. Leave at room temperature for 1-2 days. Place the bag in a controlled refrigerator/freezer at 50F for one week and then decrease to 35-40F for 2 months. Raise back to 50F for one week and then room temperature for one day prior to the germination process. 3. Germination: In vitro Murashuge Skoog supplemented with GA3 or directly on soilless medium (which would be better)? If using the MS medium, transplant to undetermined soilless growing mix once sprouted. 4. Hardening off: Not sure what the protocols will be yet. Goal will be transplant plugs in the Spring into several fen wetlands.
Any advice is much appreciated!
Submitted December 30, 2017 at 02:59AM by thatsranunculus http://ift.tt/2Ef3ms8 botany
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